Coding

Part:BBa_K1766014:Design

Designed by: Pontus Höjer   Group: iGEM15_Stockholm   (2015-09-14)


EnvZ osmoregulatory histidine kinase from E.coli.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 316
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 316
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 316
    Illegal BglII site found at 236
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 316
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 316
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Illegal restirction site EcorI present in original sequence at 315-321 bp. Site-directed mutagenesis, exchanging A317G, was appempted using primers F1 and R1 but was unsuccesful.
F1: cactatgagttcttaagccatcagatggcgc
R1: gcgccatctgatggcttaagaactcatagtg



Source

EnvZ was isolated from E.coli K12.

References